Flow cytometry identified abnormal antigen expression on myeloid cells including decreased CD10, increased CD13, abnormal HLA-DR (small subset), and CD117 (small subset) expression.
Approximately 10% myeloblasts were detected by flow cytometry, expressing CD34, CD13, CD117, MPO, and HLA-DR.
Dysplastic neutrophils on the peripheral blood smear
The core biopsy shows hypercellular marrow with erythroid hyperplasia, left-shifted myeloid population and atypical megakaryocytes
CD34 stains ~5% immature cells, CD61 highlights the atypical megakaryocytes on the core biopsy
Representative morphologic features from bone marrow aspirate smears (100X). Erythroid precursors show megaloblastoid changes, nuclear budding, nuclear irregularity, and nuclear bridging. Myeloid precursors show dysplastic features including hypogranulation and hyposegmentation.
Representative morphologic features from bone marrow aspirate smears (100X). Erythroid precursors show megaloblastoid changes and nuclear budding.
Representative morphologic features from bone marrow aspirate smears (100X). Nuclear bridging is clearly visible in this picture (arrow).
Representative morphologic features from aspirate smears (100X). Myeloid precursors show dysplastic features including hypogranulation and hyposegmentation.
Representative morphologic features from aspirate smears (100X). Megakaryocytes are small and hypolobated or monolobated.
Chromosome analysis revealed inv (3) (p21q26), del (5) (q13q33), del (7) (q22q34), del(13) (q13q22), and -14 in 16/20 metaphase cells