Bone marrow aspirate: left-shifted myelopoiesis with increased blasts including occasional megakaryoblastsBone marrow aspirate: left-shifted myelopoiesis with increased blasts including occasional megakaryoblasts
Hypercellular bone marrow core biopsy with increased megakaryopoiesis. Some megakaryocytes are large with hyperchromatic nuclei as well as occasional hypolobated nucleiHypercellular bone marrow core biopsy with increased megakaryopoiesis. Some megakaryocytes are large with hyperchromatic nuclei as well as occasional hypolobated nuclei
Representative karyogram at a 400 G-band resolution depicting an interstitial deletion of the long (q) arm of chromosome 5Representative karyogram at a 400 G-band resolution depicting an interstitial deletion of the long (q) arm of chromosome 5
In a normal cell (upper left diagram), the expected FISH signal pattern is two orange probes (CSF1R) and two green probes (D5S23/D5S721). In a hybridized abnormal cell containing the 5q33-­q34 deletion (upper right and below), the one orange, two green signal pattern is observedIn a normal cell (upper left diagram), the expected FISH signal pattern is two orange probes (CSF1R) and two green probes (D5S23/D5S721). In a hybridized abnormal cell containing the 5q33-­q34 deletion (upper right and below), the one orange, two green signal pattern is observed
Patient (pictured) and control DNA were amplified by real-time PCR using a single primer set and two fluorogenically-labeled probes specific for the wild-type and V617F mutation on the JAK2 gene. Determination of allele genotype was made by examining the resulting amplification plot and corresponding CT value produced for each samplePatient (pictured) and control DNA were amplified by real-time PCR using a single primer set and two fluorogenically-labeled probes specific for the wild-type and V617F mutation on the JAK2 gene. Determination of allele genotype was made by examining the resulting amplification plot and corresponding CT value produced for each sample