Touch imprint showing numerous blasts and a few eosinophils/eosinophil precursors (DiffQuik Stain)
Lymph node with sheets of blasts
Lymph node showing sheets of blasts and a few eosinophils/eosinophil precursors
Immunostain for CD1a shows positivity in the blasts
Immunostain for CD3 shows positivity in the blasts
Immunostain for CD117 shows a few positive cells.
Immunostain for CD33 shows a few positive cells.
Immunostain for myeloperoxidase shows a few positive cells.
Immunostain for TdT is positive in the blasts in the lymph node.
Immunostain for Ki67 shows Ki67 positivity in almost 100% cells.
Bone marrow core biopsy shows sheets of eosinophils and eosinophil precursors.
Bone marrow core biopsy with areas of marked fibrosis.
Reticulin stain shows marked reticulin fibrosis.
Immunostain for CD61 on the bone marrow core shows increased megakaryocytes.
Second bone marrow aspirate shows numerous blasts.
Second bone marrow core shows sheets of blasts.
Second bone marrow core shows fibrosis.
Second bone marrow core shows strong and diffuse CD34 positivity.
Cytogenetic analysis of second bone marrow shows t(8;13).
Cytogenetic analysis of second bone marrow shows t(8;13) in second cell line.
Metaphase FISH in second marrow shows involvement of FGFR1 in t(8;13) in one cell line (breakapart probe)
Metaphase FISH in second marrow shows involvement of FGFR1 in t(8;13) in second cell line (breakapart probe)
Interphase FISH in second marrow shows involvement of FGFR1 in t(8;13) - breakapart probe was used.
Interphase FISH in lymph node with bilineal lymphoma shows FGFR1 rearrangement (breakapart probe).