Bone marrow aspirate smear showing trilineage hematopoiesis with abnormal small and hypolobated megakaryocytes (x400, Wright-Giemsa)Bone marrow aspirate smear showing trilineage hematopoiesis with abnormal small and hypolobated megakaryocytes (x400, Wright-Giemsa)
Bone marrow biopsy section showing essentially normal cellularity with no abnormal infiltrates (x40, H&E) Bone marrow biopsy section showing essentially normal cellularity with no abnormal infiltrates (x40, H&E)
Bone marrow biopsy section showing many abnormal small megakaryocytes with hypolobated nuclei (x400, H&E) Bone marrow biopsy section showing many abnormal small megakaryocytes with hypolobated nuclei (x400, H&E)
Karyotype on bone marrow aspirate showing deletion of long arm of chromosome 5 (5q-)(arrowhead)Karyotype on bone marrow aspirate showing deletion of long arm of chromosome 5 (5q-)(arrowhead)
FISH on bone marrow aspirate showing cells with 2 green and 1 red signals indicating deletion of long arm of chromosome 5 (VYSIS FISH probes to 5q31 EGR1 -red, 5p15.31 D5S7/D5S23 - green) FISH on bone marrow aspirate showing cells with 2 green and 1 red signals indicating deletion of long arm of chromosome 5 (VYSIS FISH probes to 5q31 EGR1 -red, 5p15.31 D5S7/D5S23 - green)
Selected bone marrow aspirate flow cytometry histograms showing no significant increase in blasts Selected bone marrow aspirate flow cytometry histograms showing no significant increase in blasts